Bacterial biofilms pose major treatment challenges, but introducing oxygen via calcium peroxide nanoparticles (CaO2 NP) offers a powerful strategy to boost antibiotic effectiveness. Research shows that combining CaO2 NP with tobramycin sulfate significantly enhances biofilm eradication against Pseudomonas aeruginosa in vitro. Furthermore, clinical oral studies demonstrated that this combination increases bacterial mortality to 69%, confirming the nanoparticles' strong potential as antimicrobial and antibiotic potentiators